We used quantitative reverse-transcription PCR (RT-PCR) to validate the key genes Mef2a, Klf15, Bcl2l2, Cd36, and Slc25a33, which are associated with DCM pathophysiology and found that all of them were significantly enriched in immunoprecipitation (IP) pull-down samples (Figure 4E). This evidence concerns the gene SLC25A33 and familial dilated cardiomyopathy.