These studies reported that using 5 mM MTZ to treat the Tg(zp:GFP-NTR) transgenic line zebrafish females at 28 day post-fertilization (dpf) for 2 weeks caused infertility due to complete apoptosis of their germ cells (Hu et al., 2010), whereas other study used the same method to treat the adult Tg(zpc:g4vp16/uas:nfsb-mcherry) transgenic background females and found that a large number of oocytes were killed, but 1 month later the ovaries could recover completely to regain their reproductive functions (Figure 1A) (White et al., 2011). Here, NTSR1 is linked to Infertility.