Subsequently, consistent with the prediction, the upregulated expression of NAP1L1 mRNA and protein levels was confirmed by real-time quantitative PCR (qRT-PCR), Western blot analysis of the human HCC and normal liver cell lines, and immunohistochemistry assay on the clinic human HCC tissue sections (Figures 1C–E). This evidence concerns the gene NAP1L1 and hepatocellular carcinoma.