As elevated glucose uptake, enhanced production of the glycolytic metabolites lactate and pyruvate, and elevated ATP production are features of the Warburg effect (14–16), we measured glucose uptake, secretion of lactate into the supernatant, intracellular pyruvate levels, and intracellular ATP levels in A549 and H1299 NSCLC cells subjected to pcDNA.HIF1A or pcDNA.Ctrl plasmid transfection (Figure 1A and Figure S1A). The gene discussed is HIF1A; the disease is non-small cell lung carcinoma.