We identified both miR-4666a-5p and miR-6124 as potential upstream regulators of ALYREF and eIF4A3 and identified the overexpression of eight hub genes (BIRC5, MCM2, MCM3, MCM6, NOP56, PCNA, RFC4 and SNRPD1) in HCC tissues. This evidence concerns the gene ALYREF and hepatocellular carcinoma.