The Cistrome Cancer DB uses three filtering criteria to determine putative TF–gene relationships: i) The TF should be active in a cancer type, i.e., the reads per kilobase million (RPKM) value in a cancer type must be greater than the median RPKM expression of the TF across all 29 different cancer types; ii) the RNA expression of the TF and target gene should be correlated. This evidence concerns the gene TF and cancer.