TNF and neoplasm: Typically cytotoxic cells (sometimes called Tc1 subtype) are efficient in killing tumor and pathogen-harboring cells and they have associated markers such as intracellular IFN gamma and TNFa as well as transcription factors like EOMES, T-bet and STAT-4, while other T-cell subsets, like the Tc17 with low cytotoxic activity (45).