SMAD4 and colon adenocarcinoma: In the ICI-treated COAD cohort (Figure 2A), the genes with the top 20 mutation frequencies were APC, KRAS, TP53, PIK3CA, KMT2D, ARID1A, PTPRS, RNF43, KMT2C, TCF7L2, ZFHX3, FAT1, NCOR1, SMARCA4, NF1, PTCH1, SMAD4, ARID1B, BRCA2 and CREBBP.