With further research, we found that the protein expression of p-mTOR and its downstream p-p70 S6K and p-4E-BP1 significantly increased with MHY1485 pretreatment (Figures 3C, D), while RAPA inhibited the p-mTOR, p-p70 S6K and p-4E-BP1 protein expressions regardless of the presence or absence of viral infection (Figures 4C, D). Here, TRERF1 is linked to viral infectious disease.