In consideration of the well-known involvement of retinal activated microglia, astrocytes and Muller glial cells in glaucoma, we assessed retinal Iba1 and GFAP expression, which were significantly (p < 0.05) increased after I/R injury (3-fold and 5-fold, respectively) compared to control (Figure 3B). This evidence concerns the gene AIF1 and glaucoma.