Next, western blotting and RT-PCR were carried out to test HDAC2 and PD-L1 expression in various breast cancer cells, including 3 TNBC cells (MDA-MB-231, BT-549, and 4T-1) and 2 non-TNBC cells (SKBR3, T47D). The gene discussed is CD274; the disease is breast cancer.