To study the function of HSPD1 in NSCLC cells in vitro, we analyzed the effects of its knockdown by transducing 5 different NSCLC cell lines with up to 5 independent shRNA sequences (called #44, #45, #46, #47 and #48) (Fig. 2A, Additional file 1: Fig. S1A). Here, HSPD1 is linked to non-small cell lung carcinoma.