The results indicated that there was activation of the canonical Wnt pathway in the primary AA and AW lines of parental sarcoma as these lines showed elevated protein expression of the dephosphorylated form of β-catenin, increased expression of the positive effectors CTNNB1 (β-catenin gene) and TCF4 and decreased expression of EMX genes (Fig. 2A, B). This evidence concerns the gene CTNNB1 and sarcoma.