To verify the effect of TACR2 on Wnt/β-catenin signaling transduction, we selected hormone-resistant prostate cancer cells (DU145 and PC3) and hormone-sensitive prostate cancer cells (LNCaP) and transfected them with TACR2 overexpression lentiviral vector and corresponding negative control lentiviral vector. This evidence concerns the gene TACR2 and prostate cancer.