To examine the function of DEGS2 in CRC, we established stable DEGS2-overexpressing CRC HCT116 cells with DEGS2 overexpression lentivirus and constructed a DEGS2 knockdown cell line with shRNA and confirmed the efficiency of these methods by qPCR and WB (Fig. 5a). Here, DEGS2 is linked to colorectal carcinoma.