Consistent to the first GWAS results listed above, by looking at the established leading T1D signal of each locus, the frequencies of the predisposing alleles of HLA, PTPN22 and IKZF4 were lower in the low T1D PRS cohort, while the protective allele of INS were higher in the low T1D PRS cohort. This evidence concerns the gene INS and type 1 diabetes mellitus.