Because the ALS lines showed neither a deficit in neurite outgrowth nor a difference in the percentage of skeletal muscle area (Fig. 6f) when compared to their isogenic controls, we examined the NMJs by transducing separate cultures with hSYN::EGFP at 13 weeks and labeling with α-bungarotoxin 2 weeks later. The gene discussed is RIC8B; the disease is amyotrophic lateral sclerosis.