Further, CD4+Foxp3+ T cells, known as regulatory T cells (Tregs), which can hamper effective antitumor immunity, were obviously decreased when DSM and ES-DSM were applied with hyperthermia, suggesting that tumor ICD effectively stimulated T-cell immunity and weakened the immunosuppressive effect of Tregs (Supplementary Fig. 10). Here, FOXP3 is linked to neoplasm.