Since RSL1D1, as a nucleolus-localized protein, is released to the nucleoplasm of H1299 non-small cell lung cancer cells upon nucleolar stress [20], an IF assay was performed to address whether this nucleolus-nucleoplasm translocation of RSL1D1 occurs in HCT116 CRC cells. The gene discussed is RSL1D1; the disease is non-small cell lung carcinoma.