Given that the association between HSPA8 and Iba-1 was weaker than that of HSPA8 and GFAP immunoreactivity cells, especially after 12 h of reperfusion, we emphasize that HSPA8 of the spinal astrocyte might play an essential role in the pathogenesis of ischemia and reperfusion injury. This evidence concerns the gene HSPA8 and ischemia.