Considering the HSPA8 expression colocalizes with Iba-1 immunoreactive cells following ischemia (Supplementary Figure), suggesting that the spinal microglial HSPA8 might involve the ischemia–reperfusion injury, which is consistent with the view that spinal microglial cells attribute to the neuron loss associated with ischemia–reperfusion injury. Here, HSPA8 is linked to ischemia.