The upregulation of NUCB2/NESF-1 mRNA in prostate cancer was correlated with higher Gleason scores (p < 0000.1), higher levels of preoperative prostate-specific antigen (PSA) (p = 0.0004), positive lymph node metastasis (p = 0.022) and positive angiolymphatic invasion (p = 0.0004). This evidence concerns the gene NUCB2 and Familial prostate cancer.