Furthermore, to confirm the presence of cardiac functional abnormalities, we extracted the total RNA from WT and KO mice and performed a RT-qPCR analysis to evaluate the expression levels of specific heart failure markers such as atrial natriuretic peptide (NPPA), brain or B-type natriuretic peptide (NPPB), and myosin heavy chain 7 (MYH7). The gene discussed is NPPA; the disease is heart failure.