We then chose Interleukin 6 (Il-6) and C-X-C motif ligand 1 (Cxcl-1) (as murine Interleukin 8 (Il-8) homologue) mediators, an cytokine well-known to play an important role in ARDS [23,24] as the exemplary target gene to examine and document the relevance of reference gene stability on gene normalisation. This evidence concerns the gene IL6 and acute respiratory distress syndrome.