In the present study, we analyzed the domain distribution of the most prevalent and position-sensitive missense variants in four α connexins (Cx43, Cx46, Cx40, and Cx50), including those documented to associate with inherited diseases [24,25,26,27,28] and those found in the reference genome/exome database, gnomAD [13,14]. This evidence concerns the gene GJA8 and hereditary disease.