In addition, analysis of breast cancer (BC) epithelial tissues showed that high FREM1-expressed BC tissues harbor abundant CD4+T memory cells (resting and activated), CD8+T-cells, M1 macrophages, resting dendritic cells, gamma-delta T cells (γδT), B-cells (naive and memory), plasma cells, and resting mast cells. This evidence concerns the gene CD4 and breast cancer.