We next assessed the role of LRP6 in two established human CRC cell lines exhibiting activated Wnt signaling, namely HCT116 (CTNNB1 mutation) and SW48 (APC mutation) cells, by using a doxycycline-inducible shRNA expression vector targeting LRP6 mRNA, to knockdown LRP6 protein expression. Here, APC is linked to colorectal carcinoma.