Therefore, we conducted a more precise evaluation of the role of CSNK2A1-SIRT6 in human cancer, especially in osteosarcoma, and we showed that the phosphorylation status of SIRT6 on Ser338 is important in CSNK2A1-mediated chemoresistance in osteosarcoma cells. The gene discussed is CSNK2A1; the disease is cancer.