CD28 and cancer: The subgroup analysis factors included patients’ characteristics such as cancer types (ALL vs. NHL), region (China vs. USA), age (adult ≥ 20 years old vs. child and young ≤ 30 years old), and factors related to the process of CAR-T production such as T-cell origin (autologous vs. allologous), co-stimulation domain (4-1BB vs. CD28 vs. 4-1BB combined with CD28), vector (gammaretrovirus vs. lentivirus vs. retrovirus), lympho-depletion protocol (Cy vs. Cy+Flu), and dose (105–106/kg vs. 106/kg vs. 106–108/kg).