The negative control (immune-complex SPMP-5AB blocked with Tropix i-Block buffer and stained with CD63-FITC antibodies), and the representative results of colon-specific ENV quantification in plasma samples obtained from healthy donors or CRC patients, respectively, are shown in Figure 4A. Again, the colored inserts revealed increased fluorescent signal from the beads with a higher number of attached tissue-specific ENVs in the CRC sample. Here, CD63 is linked to colorectal carcinoma.