CNGB1 and cancer: Next, a complementary approach of traditional gene ranking by differential gene expression analysis revealed CNGB1, encoding a cyclic nucleotide-gated channel that regulates intracellular cation (Ca2+) flow and downstream transduction signalling cascades, as the top ranked upregulated gene in non-responders to NAC and its expression was validated in a further additional cohort of MIBC patients and in in vitro studies of MIBC cancer cell lines.