CD4 and neoplasm: We therefore investigated the spatial locations of tumour-associated macrophages (TAMs) by assessing CD68 + cells, activated TAMs (PD–L1 + CD68 + cells), regulatory T cells (FOXP3 + CD4 + cells and activated regulatory T cells; PD–L1 + FOXP3 + CD4 + triple positive cells [45]), stratifying into tumoral and stromal locations.