Interestingly, in osteosarcoma cells under acidic conditions, we observed increased release of extracellular nanovesicles with proangiogenic activity, including urokinase-type plasminogen activator (uPA), angiopoietin-2 (Ang-2), and VEGF, as well as the presence of miRNAs related to angiogenesis, as demonstrated by the formation of tubule branches in the chorioallantoic membrane (CAM) [101], suggesting that local acidosis might be responsible for promoting neoangiogenesis. The gene discussed is PLAU; the disease is osteosarcoma.