Collectively, our study suggested that the cancer hub genes, namely, RAB31, IRAK3, OBSCN, LIN9, TNPO2, and SEC16B, are crucial biomarkers of the immuno-oncology context of the tumor microenvironment, tumor staging, prognosis, and therapy response in liver, lung, stomach prostate, and colorectal cancer. This evidence concerns the gene SEC16B and colorectal cancer.