To address this point, by performing a stable NRP-1 depletion in the HNSCC cell lines HN6 and CAL33, carrying EGFR amplification and wild-type status, respectively, an increased sensitivity to chemotherapy was observed in both the HNSCC cells, which is in support of a role for NRP-1 in cisplatinum sensitivity. This evidence concerns the gene EGFR and head and neck squamous cell carcinoma.