VIM and hepatocellular carcinoma: As shown in Figure 2, compared with naïve cells, sorafenib-resistant HCC cells had significantly higher expression levels of YAP, as well as of IGF-1R; IGF-1; and the mesenchymal-related proteins VIMENTIN, SNAIL1, and N-CAD, both at the transcriptional level, as shown by mRNA analysis (Figure 2A and Figure S1), and at the protein level, as shown by Western blotting analysis (Figure 2B).