Here, we investigated a series of 59 human cutaneous melanoma samples with B cell infiltration to determine the spatiotemporal expression of the two most prominent cytokines with opposing immunoregulatory activities, namely lymphotoxin alpha (LTA/TNFSF1) and Interleukin-10 (IL-10), in different antigen-experienced B cell subpopulations. This evidence concerns the gene IL10 and cutaneous melanoma.