Following the fusion of SP2/0 myeloma cells with spleen lymphocytes from mice vaccinated with recombinant Ov-TSP-2-LEL, the fused cells were isolated and cloned using the semi-solid medium ClonaCellTM-HY Medium D. After incubation for 14 days at 37 °C, the hybridoma cells were harvested from the semi-solid medium. Here, THBS2 is linked to plasma cell myeloma.