Similarly, the PTX3 protein was secreted and dosed in the conditioned medium and had an incremental yet not significant trend in the course of the infection (Figure 6C), thus reflecting the gene transcription profile reported in Figure 6B. No changes over time were observed in the concentration/activity of ALP, OPG, PTX3, and TNF-α (which was undetectable in the 3D setting) in the supernatant of 2D cultures (Figure 6C). The gene discussed is TNFRSF11B; the disease is infection.