To overcome this, the approach of saturation target biopsy (STB) has been proposed in which two target cores, two cores in the target sector and two cores from the adjacent sectors are taken, and can achieve >90% detection of Gleason score >7 PCa.67 When compared to extended prostatic biopsy a recent meta-analysis of 11,997 patients undergoing TRUS-guided prostate biopsy showed that STB had a significant advantage in biopsy-naive males, particularly those with PSA <10 ng ml−1, prostate volume >40 cc or PSA density <0.25 ng ml−1/cc.57 This evidence concerns the gene KLK3 and posterior cortical atrophy.