Collectively, our findings confirm and expand these previous observations by showing that plasma levels of primary BAs and secondary BAs (especially TCDCA, TDCA, HDCA, GDCA, GLCA and DCA—most of which are conjugated BAs) were significantly higher in patients with T2DM (treated or not with metformin) than in subjects without T2DM, even after adjusting for age, sex, adiposity measures, serum ALT levels, hypertension and use of statins (that are able to modulate plasma BA levels by several mechanisms [13]). This evidence concerns the gene GPT and Hypertension.