CLDN1 and Glucose intolerance: Thus, overall, the factorial ANOVA analysis performed in this study suggests that the phenotypic effects are due to an interaction between genotype, diet and microbiome composition, supporting the differences between WT and PASK−/−, and suggesting a protective effect provided by PASK deficiency, which could be overcome by the microbiota in the lower cluster in the case of claudin-1 expression, weight gain, or glucose intolerance, but not triglyceride accumulation.