CASP3 and neoplasm: After treatment with menin-ASO (200 nM) for 72 h, the levels of cleaved caspase-3 (active form that cleaves PARP) and cleaved-PARP, the hallmark of apoptosis (inactive form, leading to impaired DNA repair) were remarkably increased compared with the control samples in both Hs 578T and MCF-10A cells (Figure 4d), confirming that menin silencing by ASO21 can induce tumor cell apoptosis.