When the cells were subjected to flow cytometry analysis after stained with Annexin V-PI double staining, the GBM cells co-treated with 25 nM of miRNA and 10 μM of TMZ presented nearly twice apoptotic cells (62.6 ± 0.3%) compared to miR-138 (32.9 ± 0.3%) or TMZ alone (32.0 ± 0.3%) (Figure 2B,C, p < 0.001). This evidence concerns the gene ANXA5 and glioblastoma.