Evidence obtained from SK-OV-3 and OVCAR-3 ovarian cancer cell lines showed that high level of NO donors, including spermine nitric oxide complex hydrate (SPER/NO) and diethylenetriamine nitric oxide adduct (DETA/NO), induced apoptosis of both ovarian cancer cell lines accompanied with decreasing of STAT3 and Akt protein phosphorylation. This evidence concerns the gene AKT1 and ovarian cancer.