Mechanistically, phosphorylation of Fbx4 on ser12 induces its association with 14-3-3ε, which promotes the Fbx4 E3 ligase activity [99], suggesting Fbx4 mutations, or 14-3-3ε expression, may serve as biomarkers for colon cancer. The gene discussed is FBXO4; the disease is malignant colon neoplasm.