While in male C57BL/6J mice, fed with HFD and injected with DEN, green tea extract (2% in diet) was observed to prevent the hepatic oncogenesis by inhibiting carcinogenic cascades related to NASH-related HCC, as indicated by the attenuated the frequency of proliferating cell nuclear antigen-positive hepatocytes, the decreased mRNA expressions of cyclin D1, MIB E3 ubiquitin protein ligase 1, oncostatin M, Ki-67, CD130, c-Fos, c-Myc, and survivin, and the increased apoptotic protease activating factor 1 mRNA [146]. The gene discussed is CCND1; the disease is hepatocellular carcinoma.