To further corroborate the role of G6PD activation in Pol ι-induced ESCC proliferation, a known G6PD inhibitor termed polydatin, a natural molecule found in Polygonum cuspidatum, was used to restrain the G6PD activity in Pol ι overexpressed TE-1 cells and wild type KYSE-150 cells. The gene discussed is G6PD; the disease is esophageal squamous cell carcinoma.