In summary, this research demonstrated that the administration of Nec-1 inhibited an autophagy-like cell death that may play an important role during delayed reperfusion injury after myocardial ischemia, and it significantly improved cardiac function after myocardial ischemia and reperfusion, suggesting that antinecroptosis treatment may improve the clinical outcomes of patients with ischemic heart disease. This evidence concerns the gene PCSK1 and myocardial ischemia.