Accordingly, the aims of the present experiment were (1) to investigate whether administration of Nec-1, a specific inhibitor of necroptosis, may reduce autophagy-like cell death induced by MI/R and thus contribute to its infarct reduction and myocardial functional recovery after reperfusion in vivo, and, if so, (2) to determine the influence of myoarchitectonic necessary changes on antinecroptosis effects associated with the administration of Nec-1 in the setting of myocardial ischemia and reperfusion. This evidence concerns the gene PCSK1 and myocardial ischemia.