And by HE staining to observe the influence of indicators such as the structure of the rat's colon, the changes of serum and midgut neurotransmitters 5-HT, VIP, and NO were determined and regulating the number of ICC through c-kit/SCF signal pathway to explore the mechanism of NSKP in moistening intestines and defecation. This evidence concerns the gene VIP and intrahepatic cholangiocarcinoma.