In this paper, we have investigated the cellular role of the largest intron in the PRKN gene, which is located in a highly fragile CFS (Wilson et al., 2015; Okamoto et al., 2018; Pentzold et al., 2018; Voutsinos et al., 2018). The gene discussed is PRKN; the disease is myalgic encephalomeyelitis/chronic fatigue syndrome.